TABLE 2.
Primer sequences used for gene expression analysis
| Target (GenBank accession no.)a | Forward primer (5′–3′) | Reverse primer (5′–3′) | Product length (bp) |
|---|---|---|---|
| Human ACE2 (NM_001371415.1) | AGAAAGCAGTCTGCCATCCC | GCTGTCAGGAAGTCGTCCAT | 97 |
| Mouse Ace2 (NM_001130513.1) | GTCATGGATGCGCTTTGGAT | CTTGGGTTGGGCACTGCTTA | 88 |
| Tmprss2 (NM_015775.2) | TGACGGGGTAGCACATTGTC | CACACGGGATACCAGGCTTT | 117 |
| Ccl2 (NM_011333.3) | TGACCCCAAGAAGGAATGGG | ACCTTAGGGCAGATGCAGTT | 104 |
| Ccl3 (NM_011337.2) | ACATTCCTGCCACCTGCATA | GAAGAGTCCCTCGATGTGGC | 74 |
| Ccl4 (NM_013652.2) | CTAACCCCGAGCAACACCAT | CCATTGGTGCTGAGAACCCT | 97 |
| Cxcl10 (NM_021274.2) | CTGAGTCCTCGCTCAAGTGG | GTCGCACCTCCACATAGCTT | 69 |
| Gm-csf (NM_009969.4) | GCCAGTTCTTGGAAGGGCTTAT | ATCTCCTGGCCCTTATCAGCTA | 99 |
| Il-2 (NM_008366.3) | GAAACTCCCCAGGATGCTCA | CGCAGAGGTCCAAGTTCATCT | 99 |
| Il-6 (NM_031168.2) | GGGACTGATGCTGGTGACAA | ACAGGTCTGTTGGGAGTGGT | 90 |
| Tnfα (NM_013693.3) | AGCCGATGGGTTGTACCTTG | ATAGCAAATCGGCTGACGGT | 99 |
| Actb (NM_007393.5) | CACTGTCGAGTCGCGTCC | TCATCCATGGCGAACTGGTG | 89 |
| Gusb (NM_001357025.1) | GGCGATGGACCCAAGATACC | ACCCTTGGGATACCACAACT | 84 |
| Rpl13a (NM_001357025.1) | CCCACAAGACCAAGAGAGGC | CACCATCCGCTTTTTCTTGTCA | 92 |
a
Ace2/ACE2, angiotensin converting enzyme 2; Tmprss2, transmembrane serine protease 2; Ccl, C-C motif chemokine ligand; Cxcl10, C-X-C motif chemokine ligand 10; Gm-csf, granulocyte-macrophage colony-stimulating factor; Il, interleukin; Tnfα, tumor necrosis factor α; Actb, actin, beta; Gusb, glucuronidase beta; Rpl13a, ribosomal protein L13a.