TABLE 1.
IVIG lots containing IgGs specific for a panel of peptides and a glycan epitope that are targeted by protective mouse mAbs in mouse models of disseminated candidiasisa
| IVIG lot | Titer against Candida cell surface or glycan epitope |
||||
|---|---|---|---|---|---|
| Fbab | Met6c | β-1,2-Mannotriosed | Hwp1e | Pgk1e | |
| A (batch 1741) | 512,000 | 16,000 | 32,000 | 16,000 | 6,400 |
| B | 128,000 | 16,000 | 8,000 | 16,000 | 6,400 |
| C | 4,000 | 2,000 | 32,000 | 16,000 | 6,400 |
| D | 32,000 | 2,000 | 8,000 | 8,000 | 6,400 |
| E | 4,000 | 2,000 | 8,000 | 2,000 | 4,000 |
| F | 4,000 | 2,000 | 8,000 | 2,000 | 4,000 |
| G | 64,000 | 2,000 | 32,000 | 8,000 | 64,000 |
| H | 4,000 | 2,000 | 8,000 | 2,000 | 4,000 |
| I | 4,000 | 2,000 | 8,000 | 2,000 | 4,000 |
| J | 32,000 | 4,000 | 8,000 | 8,000 | 4,000 |
| K (batch 1499) | 4,000 | 2,000 | 8,000 | 2,000 | 4,000 |
| L | 4,000 | 4,000 | 8,000 | 2,000 | 4,000 |
| M | 8,000 | 2,000 | 8,000 | 8,000 | 4,000 |
| N | 4,000 | 2,000 | 8,000 | 2,000 | 4,000 |
| O | 4,000 | 2,000 | 8,000 | 2,000 | 4,000 |
| P (batch 1743) | 32,000 | 16,000 | 32,000 | 8,000 | 6,400 |
| Q | 4,000 | 2,000 | 8,000 | 2,000 | 4,000 |
| R | 4,000 | 2,000 | 8,000 | 4,000 | 4,000 |
A panel of Candida cell surface peptides and a glycan epitope were identified and targeted by protective mAbs against invasive candidiasis in mice. Eighteen lots of commercially obtained IVIG samples (lots A to R; ADMA Biologics) were screened for IgGs specific for the identified protective epitopes, and antibody endpoint titers were determined by ELISAs. Specific IgGs recognizing the five selected epitopes were detected in all 18 lots of IVIG. ELISA data demonstrated that there are substantial lot-to-lot differences in the epitope-specific IgG antibody levels. Fba, fructose bisphosphate aldolase; Hwp1, hyphal wall protein 1; Pgk1, phosphoglycerate kinase 1; Met6, methyltetrahydropteroyltriglutamate 6.