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. 2023 Feb 21;20:6. doi: 10.1186/s12950-023-00331-4

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Effect of i.n. dsRNA administration on BALF cellular infiltration in mice. dsRNA (100 µg) or saline were i.n. administered to C57Bl/6J, C57Bl/6N or BALB/c mice once per day for three consecutive days, and cell concentration (cells/ml) (A) and cellular composition (%) (B) were determined in BALF. Representative pictures of BALF composition are shown in (C) (100X magnification). Orange arrows depict lymphocytes, purple arrows depict neutrophils and green arrows depict macrophages. Scale bars indicate 100 µm. Data are presented as mean ± SD. Differences between groups were tested using a 2way ANOVA with Tukey’s multiple comparisons test. P-values of < 0.05 are indicted in the graphs, each data point represents one mouse from one experiment (n = 4–5 per group)