Fig. 4.

The effect of i.n. dsRNA administration on gene expression of TLR3, MDA5 and RIG-I. dsRNA (100 µg) or saline were i.n. administered to BALB/c, C57Bl/6J or C57Bl/6N mice once per day for three consecutive days, and gene expression of TLR3 (A), MDA5 (B), and RIG-I (C) was determined by RT-qPCR in lung homogenates. Data are presented as mean ± SD, as relative to reference gene β-actin and fold change compared to the mean of saline within each strain. Differences between groups were tested using a 2way ANOVA with Tukey’s multiple comparisons test. P-values of < 0.05 are indicted in the graphs, each data point represents one mouse from one experiment (n = 5 per group)