Fig. 4.

Developmental staging of B. stoltei for RNA-seq. Classification of nuclear morphology into stages is according to previous descriptions (6). Nuclear events occurring before and up to, but not including fusion of the gametic nuclei (syngamy) are classified into sixteen stages indicated by roman numerals. These are the pre-gamic stages of conjugation where the MICs undergo meiosis and the haploid products of meiotic MICs are exchanged between the conjugating cells. Stages after syngamy are classified into ten stages as in Fig. 1. Illustration of various cell stages (adapted from ref. 39). Stacked bars show the proportion of cells at each time point at different stages of development, preceded by the number of cells inspected (n).