Fig. 5.
Absence of PCNA ubiquitination and REV1 is lethal in adult mice. A, Relative number of indicated cells of indicated genotypes in the BM of adult mice SM mice. B, Inducible inactivation of DDT in adult mice: Conditional ablation of a floxed PCNA allele in mice that feature a non-floxed PCNA-K164 or K164R allele results in the generation of PCNA-ub-dependent DDT-deficient mice, and "WT" controls. In the absence of functional REV1, this tamoxifen-inducible deletion results in the generation of DM mice. WT controls feature a heterozygous deletion of Rev1. C, Activation of the CreERT2 in the mice by intraperitoneal injection of tamoxifen results in the genetic rearrangement of the confetti construct present in the mice. This allows for long-term tracing of deleted cells in mice by determining the frequencies of cells expressing GFP, RFP, YFP, or CFP. D, (Top) The graphs indicate specific cells counts per 1 × 10−6 of single cells, after twp tamoxifen injections (right part of the graph), or after oil injection (left part of the graph). Data were pooled from two independent experiments. (Bottom) The graphs indicate the percentage of confetti-positive cells (expressing one or more colors of the confetti construct), after two tamoxifen injections after 2 and 4 wk (middle and right part of the graph), or after oil injection (left part of the graph). Data were pooled from two independent experiments. P values were calculated using an unpaired t test. From left to right, n = 4, 6, 5, 6, 5, 6.