Fig. 4.
Structure and dynamics of Sa1V90T as a function of temperature. (A) 2D 1H-15N HSQC spectrum of Sa1V90T at 30 °C with backbone amide assignments (Left) and the corresponding ensemble of 10 lowest energy α/β-plait structures (right). (B) 2D 1H-15N HSQC spectrum of Sa1V90T at 5 °C with backbone amide assignments (left) and the ensemble of 10 lowest energy 3α structures (Right). (C) Plot of 15N-R1 and -R2 rates, and {1H}-15N steady-state heteronuclear NOE versus residue for Sa1V90T in the 3α state at 5 °C (red) and α/β-plait form at 30 °C (blue).