Fig. 7.
Kinetics of fold interconversion between α/β-plait and 3α states using ZZ-exchange NMR. (A) Spectra showing the buildup of exchange crosspeaks (blue and magenta) and decay of autopeaks (red and orange) for G58 at several mixing times at 17 °C. (B) The time-dependent buildup of crosspeaks was determined for three residues (Q16, G34, and G58) using the composite peak intensity ratio (Ξ) fit equation (Eq. 3). The average curve fit is shown in black. (C) Arrhenius analysis of activation energy, Ea. The average exchange rate constants from residues Q16, G43, and G58 were obtained from ZZ-exchange experiments at 10, 17, and 24 °C. The logarithm of the rate constants was plotted against inverse temperature and Ea was obtained from the slope of the linear fit equation (Eq. 4).