Fig. 1.
Identification of stable nonvesicular RNAs. (A and B) Northern blot of different rRNAs and tRNAs after incubating purified RNA (A) or ribosomes (B) from human cells in 10% FBS. (C and D) Northern blot of 5’ tRNAGlyGCC (D, Left) or 5′ 28S rRNA-derived fragments (D, Right) in extracellular nonvesicular fractions purified by density gradients (C). U2-OS cells were treated or not with NaAsO2 (ARS) before collecting the CCM. (E) Read coverage in small RNA-seq data of extracellular ribosomes [from Tosar et al. (33)], revealing enrichment of 40-nt 5′-derived small RNAs among all other 28S rRNA-derived fragments.