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Nicked tRNAs are a source of nonvesicular single-stranded tRNA halves that can be taken up by recipient cells. (A) Synthetic 5′ biotinylated RNAs were added to the media of MCF-7 cells, and oligonucleotide uptake was confirmed by confocal microscopy using APC-coupled streptavidin (B and C) or by SL-RT-qPCR (D). (E) Proposed model.Red circles: phosphate groups. Yellow circles: OH groups.
