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. 2023 Jan 18;120(4):e2218118120. doi: 10.1073/pnas.2218118120

Fig. 3.

Fig. 3.

CBX5 loss results in E2F1-induced transcriptional activation of the BIRC5 gene. (A) Relative expression of BIRC5 mRNA in the indicated LUAD cell lines expressing an NS or CBX5 shRNA, as measured by qRT-PCR. (B) Immunoblot analysis measuring expression of BIRC5 and CBX5 in the indicated LUAD cell lines expressing an NS or CBX5 shRNA. (C) Immunoblot analysis measuring expression of CBX5, E2F1, and BIRC5 in the indicated LUAD cell lines expressing an NS or CBX5 shRNA alone, or in combination with an NS or E2F1 shRNA. (D) Relative enrichment of E2F1 on the BIRC5 or ACTINB promoters measured by the CUT&RUN assay. (E) Immunoblot analysis measuring expression of E2F1 and BIRC5 in the indicated parental or EGFRi-resistant LUAD cell lines. Data are presented as the mean ± SEM. ns = not significant; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.