Figure 2.

Pfs230-C1 mAbs show a range of functional activity in standard membrane feeding assay

(A) mAbs were tested at 100 μg/mL in parallel in the same membrane feeding experiment. Transmission-reducing activity (TRA) is reduction in oocyst intensity, compared with negative control, and error bars indicate 95% confidence intervals. Antibodies are grouped and colored by domain specificity and epitope bin. No binning data are available for RUPA-103, but it is genetically similar to RUPA-97 (VH and VL amino acid sequence homology >95%) and therefore grouped with other antibodies from bin I.

(B) mAbs that showed >80% TRA in (A) were tested at 100 μg/mL in the presence (+C) or absence (−C) of complement in two independent membrane-feeding experiments, except for RUPA-32 and RUPA-97 that were tested in a single experiment due to limited mAb availability. The complement-dependent rodent α-Pfs230 mAb 2A2²⁴ was tested at 10 μg/mL and used as positive control. Bars are estimates of the mean from two independent standard membrane feeding experiments, and error bars represent the 95% confidence intervals.

(C) Titrations of active mAbs were tested in multiple independent membrane feeding assays in the presence of complement to calculate IC₈₀ values, using linear regression analysis. IC₈₀ values with 95% confidence intervals are shown next to graph. Raw SMFA data can be found in Table S2.