Figure 5.

Epitope delineation of known Pfs230-D1-binding antibodies

(A) Heatmap of binding competition experiments. High signal responses for the second binding event (white) represent low competition, whereas low signal responses (black) correspond to high competition.

(B) Superposition of all known structures of mAbs bound to Pfs230 D1. RUPA-97 serves as an exemplar of the bin I mAbs from natural infection. Binding affinity measured by biolayer interferometry is also indicated in Table S5. PDB IDs for Fab 4F12 and scFv LMIV230-01 are 6OHG and 7JUM, respectively.

(C) mAbs from different epitope bins were tested in multiple independent SMFAs to calculate IC₈₀ values (μg/ml), using linear regression analysis. IC₈₀ values with 95% confidence intervals are shown in inset. TRA, transmission-reducing activity (reduction in oocyst intensity, compared with negative control). Colors correspond to (B). Raw SMFA data can be found in Table S2.