Potent transmission-blocking monoclonal antibodies from naturally exposed individuals target a conserved epitope on Plasmodium falciparum Pfs230

. 2023 Feb 14;56(2):420–432.e7. doi: 10.1016/j.immuni.2023.01.013

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies

RUPA-32 (IgG1)	This paper	N/A
RUPA-33 (IgG1)	This paper	N/A
RUPA-38 (IgG1)	This paper	N/A
RUPA-39 (IgG1)	This paper	N/A
RUPA-55 (IgG1)	This paper	N/A
RUPA-62 (IgG1)	This paper	N/A
RUPA-73 (IgG1)	This paper	N/A
RUPA-96 (IgG1)	This paper	N/A
RUPA-97 (IgG1)	This paper	N/A
RUPA-103 (IgG1)	This paper	N/A
RUPA-115 (IgG1)	This paper	N/A
RUPA-126 (IgG1)	This paper	N/A
RUPA-133 (IgG1)	This paper	N/A
RUPA-135 (IgG1)	This paper	N/A
RUPA-136 (IgG1)	This paper	N/A
RUPA-137 (IgG1)	This paper	N/A
LMIV230-01 (IgG1)	Coelho et al.¹⁸	N/A
LMIV230-02 (IgG1)	Coelho et al.¹⁸	N/A
15C5 (IgG1)	Lee et al.²³	N/A
hu4F12 (IgG1)	MacDonald et al.¹⁴	N/A
TB31F (IgG1)	Kundu et al.⁵⁸	N/A
hu2A2 (IgG1)	This paper	N/A
RUPA-32 (Fab)	This paper	N/A
RUPA-38 (Fab)	This paper	N/A
RUPA-55 (Fab)	This paper	N/A
RUPA-97 (Fab)	This paper	N/A
LMIV230-01 (Fab)	Coelho et al.¹⁸	N/A
LMIV230-02 (Fab)	Coelho et al.¹⁸	N/A
15C5 (Fab)	Lee et al.²³	N/A
hu4F12 (Fab)	MacDonald et al.¹⁴	N/A
Alexa Fluor® 488 AffiniPure F(ab’)₂ fragment goat anti-human IgG, Fcγ fragment specific	Jackson Laboratories	Cat#109-546-098
AffiniPure Rabbit Anti-Human IgG, Fcγ fragment specific	Jackson Laboratories	Cat#309-005-008
2544 (IgG1)	McLeod et al.⁵⁹	N/A
399 (IgG1)	Pholcharee et al.⁶⁰	N/A
anti-Human IgG-HRP	ThermoFisher	Cat# 31412
anti-human IgG-AF488	Invitrogen	Cat# A-11013

Biological samples

PBMCs and plasma from Ugandan donor	Kamya et al.²²	N/A
PBMCs and plasma from Dutch expatriate	Stone et al.²¹	N/A

Chemicals, peptides, and recombinant proteins

Pfs230 C1	Lee et al.²³	N/A
Pfs230 Pro	Singh et al.⁶¹	N/A
Pfs230 D1+ (C terminal 6x STREP II tag)	This paper	N/A
Pfs230 D1+ (C terminal 6x HIS tag)	This paper	N/A
Pfs230 D1+ (N719S; C terminal 6x HIS tag)	This paper	N/A
Pfs230 D1+ (K716N; C terminal 6x HIS tag)	This paper	N/A
Pfs230 D1+ (V632A; C terminal 6x HIS tag)	This paper	N/A
Pfs230 D1+ (E612K; C terminal 6x HIS tag)	This paper	N/A
Pfs230 D1+ (T602K; C terminal 6x HIS tag)	This paper	N/A
Pfs230 D1+ (A583T; C terminal 6x HIS tag)	This paper	N/A
GIBCO™ FreeStyle™ 293 Expression Medium	Thermo Fisher Scientific	Cat#12338026
FectoPRO DNA Transfection Reagent	VWR	Cat#10118-444
DyLight™ 488 NHS Ester	Thermo Fisher	Cat#46403
DyLight™ 650 NHS Ester	Thermo Fisher	Cat#62265
Pierce IgG elution buffer	Thermo Fisher	Cat#21004
10X HBSTE running buffer	Carterra	Cat#3630
0.1 M MES	Carterra	Cat#3625
10 mM sodium acetate buffer	Carterra	Cat#3801
10 mM glycine pH 2.0	Carterra	Cat#3640
Sulfo–N-hydroxysuccinimide (sulfo-NHS)	Thermo Fisher	Cat#24510
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)	Thermo Fisher	Cat#22980

Critical commercial assays

Ni-NTA biosensors	ForteBio	Cat#18-5102
Anti-human Fab-CH1 biosensors	ForteBio	Cat#18-5125
HC30M chip	Carterra	Cat#4279

Deposited data

Crystal structure of Pfs230 domain 1 bound by RUPA-32 Fab	This paper	PDB: 7UVH
Crystal structure of Pfs230 domain 1 bound by RUPA-38 Fab	This paper	PDB: 7UVO
Crystal structure of Pfs230 domain 1 bound by RUPA-55 Fab	This paper	PDB: 7UVI
Crystal structure of Pfs230 domain 1 bound by RUPA-97 and 15C5 Fabs	This paper	PDB: 7UVQ
Crystal structure of Pfs230 domain 1 bound by LMIV230-02 Fab	This paper	PDB: 7UVS

Experimental models: Cell lines

FreeStyle™ 293-F Cells	Thermo Fisher Scientific	Cat#R79007

Experimental models: Organisms/strains

Parasite: P. falciparum ; NF54 strain	Ponnudurai et al.⁶²	N/A
Mosquito: Anopheles stephensi (Nijmegen strain)	Ponnudurai et al.⁶²	N/A

Recombinant DNA

pcDNA3.4_RUPA-32 (Fab)	This paper	N/A
pcDNA3.4_RUPA-38 (Fab)	This paper	N/A
pcDNA3.4_RUPA-55 (Fab)	This paper	N/A
pcDNA3.4_RUPA-97 (Fab)	This paper	N/A
pcDNA3.4_LMIV230-01 (Fab)	Coelho et al.¹⁸	N/A
pcDNA3.4_LMIV230-02 (Fab)	Coelho et al.¹⁸	N/A
pcDNA3.4_15C5 (Fab)	Lee et al.²³	N/A
pcDNA3.4_hu4F12 (Fab)	MacDonald et al.¹⁴	N/A
pcDNA3.4_RUPA-32 (Fab)	This paper	N/A
pcDNA3.4_RUPA-38 (Fab)	This paper	N/A
pcDNA3.4_RUPA-55 (Fab)	This paper	N/A
pcDNA3.4_Pfs230 D1+ (C terminal 6x STREP II tag)	This paper	N/A
pcDNA3.4_Pfs230 D1+ (C terminal 6x HIS tag)	This paper	N/A
pcDNA3.4_Pfs230 D1+ (N719S; C terminal 6x HIS tag)	This paper	N/A
pcDNA3.4_Pfs230 D1+ (K716N; C terminal 6x HIS tag)	This paper	N/A
pcDNA3.4_Pfs230 D1+ (V632A; C terminal 6x HIS tag)	This paper	N/A
pcDNA3.4_Pfs230 D1+ (E612K; C terminal 6x HIS tag)	This paper	N/A
pcDNA3.4_Pfs230 D1+ (T602K; C terminal 6x HIS tag)	This paper	N/A
pcDNA3.4_Pfs230 D1+ (A583T; C terminal 6x HIS tag)	This paper	N/A

Software and algorithms

PRISM Graphpad	GraphPad Software, LLC	https://www.graphpad.com/scientific-software/prism/
Phenix	Adams et al.⁶³	http://www.phenix-online.org/
Aimless	Evans et al.⁶⁴	https://www.mrc-lmb.cam.ac.uk/harry/pre/aimless.html
XDS	Kabsch et al.⁶⁵	https://xds.mr.mpg.de/html_doc/downloading.html
Coot	Emsley et al.⁶⁶	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
PyMOL	Schrödinger, LLC.	The PyMOL Molecular Graphics System, v2.3.4.
Bcftools	Li et al.⁶⁷	https://samtools.github.io/bcftools/bcftools.html
RoseTTAFold	Baek et al.²⁷	https://robetta.bakerlab.org/collabfor
AlphaFold2	Jumper et al.²⁸	https://colab.research.google.com/github/sokrypton/ColabFold/blob/main/AlphaFold2.ipynbI
I-Tasser	Yang et al.²⁹	https://zhanggroup.org/I-TASSER/
RaptorX	Xu et al.³⁰	http://raptorx.uchicago.edu/
Kinetics analysis software	Carterra	https://carterra-bio.com/applications/kinetics-software/
Epitope analysis software	Carterra	https://carterra-bio.com/applications/epitope-binning-software
MetaXpress	Molecular Devices	https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metaxpress
R (version 4.1.2)	The R Foundation	https://www.r-project.org/foundation/

Other

QuantumPlex™ optically encoded beads	Bangs Laboratories	Cat#235
Polystyrene beads	Bangs Laboratories	Cat#PC06N