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. 2023 Feb 13;41(2):288–303.e6. doi: 10.1016/j.ccell.2022.12.009

Figure 1.

Figure 1

CRISPR-Cas9 screens identify mediators of IFN-γ sensitivity and resistance

(A) Schematic of the integrated CRISPR-Cas9 and base editing screening approaches to identify genetic mediators of sensitivity and resistance to IFN-γ. Cas9 screens identify pathways and genes regulating IFN-γ response in colorectal cancer cell lines and base editing mutagenesis screens assess the functional consequence of VUS in key regulators.

(B) Gene-level volcano plots of CRISPR-Cas9 screens comparing IFN-γ-treated and control arms.

(C) gRNA-level analysis of top resistance or sensitizing genes, representing essential components of the IFN-γ pathway.

(D) Common and private genes conferring sensitivity and resistance to IFN-γ in HT-29 and LS-411N CRC cell lines identified from CRISPR-Cas9 screens. Hits were selected using MAGeCK; p < 0.05 and a false discovery rate of less than 0.05. All data are the average from two independent screens performed on separate days. See also Figure S1 and Table S1.