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. 2023 Jan 19;120(4):e2211933120. doi: 10.1073/pnas.2211933120

Fig. 3.

Fig. 3.

Metformin increases glycolysis in ileum and colon and reduces HGP in animals on HFD (A–C) Metabolomic analysis of ileum, colon and liver from the HFD and MET mice treated (50 mg/kg/day) for 10 d (one-way ANOVA and Bonferroni’s test, *P < 0.05, n = 4 to 5) (D) PTT curve and area under curve of metformin treated mice (50 mg/kg/day for 10 d) (n = 5). (E) Lactate, pH and bicarbonate levels in blood from the portal vein of HFD and MET Wistar rats. (F) Pyruvate carboxylase levels in liver from HFD and MET treated Wistar rats. (G) (1H) NMR spectra acquired at 600 MHz in D2O. (H) Acetylation of MPC1/2 in the liver of HFD and MET treated C57BL6/J mice (n = 5). (I) Acetylation of MPC1 in HuH7 cells treated with pyruvate and acetate. (J) Glucose production of Huh7 cells treated with pyruvate and acetate. (K) Glycerol tolerance test curve and area under curve of metformin treated mice (50 mg/kg/day for 10 d) (n = 5). (L) Acetylation and tissue protein levels of FBP1 in the liver of HFD and MET treated C57BL6/J mice (n = 5). (M) Acetylation of FBP1 in HuH7 cells treated with acetate.