Table 2.

Buffer recipes

Reagent	Sucrose Buffer	HEENG Buffer
Sucrose	200 mM
HEPES	20 mM	20 mM
CaCl2	0.5 mM
SDS	2%
Triton X-100	0.5%
Tween 20	0.5%
NaCl		125 mM
EDTA		1 mM
EGTA		1 mM
Glycerol		10%