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. 2023 Jan 5;324(3):F267–F273. doi: 10.1152/ajprenal.00272.2022

Figure 2.

Figure 2.

Induction of G protein-coupled receptor 116 (Gpr116) transcription in M-1 cells transfected with Foxi1. A: heterologous expression of Foxi1 induced Gpr116 transcription in M-1 cells but not in other immortalized cell lines, as revealed by RT-PCR. Endogenous expression of Gpr116 in the murine kidney is shown as a positive control. B: repeat measurement of Gpr116 expression by RT-PCR in M-1 cells transfected with pFoxi1. Endogenous expression of Foxi1 and Gpr116 in murine kidney is shown as a positive control. C: Foxi1-induced Gpr116 transcription in M-1 cells as measured by Taqman quantitative PCR. Relative expression is per Gapdh. Cycle threshold (CT) of 35 ≈ relative expression 0.0001. n = 3. Bars are means ± SE. ND, no detection (CT > 35). *P < 0.05 vs. M-1 cells. D: colocalization of Foxi1 and Gpr116 transcripts in an M-1 cell transfected with pFOXI1 as detected by RNAscope. Scale bar = 5 µm. HEK, human embryonic kidney; MDCK, Madin-Darby canine kidney.