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. 2023 Jan 20;120(4):e2218032120. doi: 10.1073/pnas.2218032120

Fig. 4.

Fig. 4.

Fast muscle-specific androgen receptor ablation increased the proportion of type I fibers in SOL muscle and altered expression of genes related to muscle fiber type. (A) Histological analysis of H&E, Oil Red O, and NADH-TR staining of SOL muscles from male 13-wk-old control and fmARKO mice. (Scale bar, 50 μm.) Black arrowheads indicate lipid droplets inside the muscle fibers of fmARKO mice. (B) Representative images of immunohistochemically stained SOL muscle from control and fmARKO mice. Fast-twitch fibers are stained green; slow-twitch fibers are blacked out. (Scale bar, 100 μm.) The mean ratios of slow-twitch fibers are shown (n = 5/group). (C) Mean fiber CSA of slow-twitch and fast-twitch fibers (n = 5/group). (D and E) Real-time PCR was used to measure gene expression of fiber-specific transcripts and muscle mass-related transcripts (n = 7 to 8/group). Error bars indicate SEM; *P < 0.05, **P < 0.01.