Table 1. Quantification of hindbrain neurons traced from the spinal dorsal horn with AAV2retro.eGFP and CTb.
Brain region | Subset | GFP only | Ctb only | GFP +Ctb + | %GFP only | %Ctb only | %GFP +Ctb + |
---|---|---|---|---|---|---|---|
RVM (all neurons) | - | 99 (11–57) | 679 (149–319) | 279 (65–134) | 9.1 (4.0–12.1) | 65.6 (59.7–73.3) | 25.3 (22.7–28.1) |
RVM (TPH2 +neurons) | All | 5 (0–4) | 269 (63–123) | 62 (12–25) | 1.5 (0–5.1) | 80.1 (76.1–83.1) | 18.5 (15.2–22.9) |
LPGi | 5 (0–4) | 79 (16–44) | 35 (7–15) | 4.2 (0–14.8) | 66.4 (54.3–77.2) | 29.4 (22.8–42.9) | |
NRM | 0 (0–0) | 143 (37–63) | 15 (4–6) | 0 (0–0) | 90.5 (87.8–94.0) | 9.5 (6.0–12.2) | |
Other | 0 (0–0) | 47 (10–21) | 12 (0–8) | 0 (0–0) | 79.7 (66.7–100) | 20.3 (0–33.3) |