Table 2. Neurons in the ventral hindbrain traced from the spinal cord with modified rabies viruses.

Number represents the total number of cells counted with the range of cells counted for each animal in parentheses. Percentages of labeled neurons expressing TPH2 are the average for all cells counted per group, with the range for each animal given in parentheses.

Labeling strategy	Rabies virus	Animals	Total GFP + cells counted in RVM	GFP neurons expressing TPH2	GFP neurons not expressing TPH2	% GFP +neurons that are TPH2+
Direct infection (5d)	SAD.RabiesΔG-eGFP (SAD-G)	3	25 (5–11)	11 (2–5)	14 (3–6)	44 (40–45)
Direct infection (7d)	SAD.RabiesΔG-eGFP (SAD-G)	3	36 (7–17)	4 (1-2)	32 (6–16)	11 (6–16)
Monosynaptic tracing	SAD.RabiesΔG-eGFP (EnvA)	4	33 (3–20)	1 (0–1)	32 (3–19)	3 (0–5)