Skip to main content
. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291

Figure 5. Ovaroid development and germ cell maturation.

Scale bars in immunofluorescence images are 40 µm. (A) Human ovaroid (F66/N.R1.G.F #4 granulosa-like cells + hPGCLCs) sections at days 2, 4, 14, and 32 of culture, stained for FOXL2 (granulosa), OCT4 (germ cell/pluripotent), and DAZL (mature germ cell). (B) Mouse ovaroid (fetal mouse ovarian somatic cells + hPGCLCs) sections stained as in panel A. (C) Fraction of OCT4+ and DAZL+ cells relative to the total (DAPI+) over time in human ovaroids and mouse xeno-ovaroids. Counts were performed at 11 time points on images from 2 replicates of human ovaroids (F66/N.R1.G.F #4 and F66/N.R2 #1 granulosa-like cells + hPGCLCs) and 1 replicate of mouse xeno-ovaroids. (D) Human ovaroid (F66/N.R2 #1 granulosa-like cells + hPGCLCs) sections at days 4 and 8 of culture, stained for SOX17 (germ cell), TFAP2C (early germ cell), and AMHR2 (granulosa).

Figure 5—source data 1. Ovaroid images (hPGCLC + F66/N.R1.G.F #4).
Figure 5—source data 2. Ovaroid images (hPGCLC + F66/N.R2 #1).
Figure 5—source data 3. Ovaroid images (hPGCLC + mouse fetal ovarian cells).
Figure 5—source data 4. Cell counts from ovaroid images.

Figure 5.

Figure 5—figure supplement 1. DAZL and OCT4 expression observed by immunofluorescence in day 16 ovaroids.

Figure 5—figure supplement 1.

Some DAZL+OCT4− cells (magenta arrows) are visible, as well as DAZL+OCT4+ cells (cyan arrows). Ovaroids are also beginning to form follicle-like morphology (yellow arrows). Scale bar is 40 µm.