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. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291

Figure 6. Ovaroid follicle formation.

Figure 6.

(A) Day 35 human ovaroid (F66/N.R1.G #7 + hPGCLC) sections stained for FOXL2, OCT4, and AMHR2. Scale bars are 40 µm. Follicle-like structures are marked with yellow triangles. (B) Whole-ovaroid view of follicle-like structures in human ovaroids (F66/N.R1.G #7). Scale bars are 1 mm. (C) Section of human ovaroid (F66/N.R1.G.F #4 + hPGCLC) at day 70 of culture, stained for FOXL2, NR2F2, and AMHR2, showing multiple small follicles (yellow triangles) consisting single layers of FOXL2+AMHR2+ cells. NR2F2+ cells are interspersed between these. Scale bars are 100 µm. (D) Section of human ovaroid (F66/N.R2 #1 + hPGCLC) at day 70 of culture, stained for FOXL2, NR2F2, and AMHR2, showing an antral follicle consisting of FOXL2+AMHR2+ granulosa-like cells arranged in several layers around a central cavity. NR2F2 staining is visible outside of the follicle (marked ‘Stroma’). Scale bars are 100 µm.