Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus, female) |
CD-1 | Charles River Labs | RRID:IMSR_CRL:022 | Used for fetal ovary isolation |
| Strain, strain background (Mus musculus, female) |
BALB/c | Charles River Labs | RRID:IMSR_APB:4790 | Used for adult ovary isolation |
| Cell line (Homo sapiens, female) |
F3 iPSC | ATCC | ATCC-BXS0116 | |
| Cell line (Homo sapiens, female) |
F66 iPSC | Other | Previously derived in-house from the NIA Aging Cell Repository fibroblast line AG07141 using Epi5 footprint- free episomal reprogramming |
|
| Cell line (Homo sapiens, female) |
F2 PGCLC | Kobayashi et al., 2022; PMID:35148847 | PMID:35148847 | Derived from the ATCC- BXS0115 hiPSC line |
| Recombinant DNA reagent |
pHDR-FOXL2-T2A- tdTomato-PuroTK |
This paper | RRID:Addgene_192892 | For engineering reporter hiPSCs |
| Recombinant DNA reagent |
pX330-FOXL2-cterm | This paper | RRID:Addgene_192893 | For engineering reporter hiPSCs |
| Recombinant DNA reagent |
Super PiggyBac Transposase Expression Vector |
System Bioscience | Cat:PB210PA-1 | |
| Recombinant DNA reagent |
pPB-cT3G-cERP2-TCF21 | This paper | RRID:Addgene_192894 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-JUN | This paper | RRID:Addgene_192895 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-GATA4 | This paper | RRID:Addgene_192896 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-CEBPD | This paper | RRID:Addgene_192897 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-EMX2 | This paper | RRID:Addgene_192898 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-MYC | This paper | RRID:Addgene_192899 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-NR2F2 | This paper | RRID:Addgene_192900 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-FOSB | This paper | RRID:Addgene_192901 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-KLF2 | This paper | RRID:Addgene_192902 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-ZBTB16 | This paper | RRID:Addgene_192903 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-YBX1 | This paper | RRID:Addgene_192904 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-LHX1 | This paper | RRID:Addgene_192905 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-WT1 | This paper | RRID:Addgene_192906 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-JUNB | This paper | RRID:Addgene_192907 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-KLF4 | This paper | RRID:Addgene_192908 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-KLF6 | This paper | RRID:Addgene_192909 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-HOXC9 | This paper | RRID:Addgene_192910 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-ATF4 | This paper | RRID:Addgene_192911 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-PPARG | This paper | RRID:Addgene_192912 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-EGR1 | This paper | RRID:Addgene_192913 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-FOXL2 | This paper | RRID:Addgene_192914 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-NR5A1 | This paper | RRID:Addgene_192915 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-TSC22D3 | This paper | RRID:Addgene_192916 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-MAFF | This paper | RRID:Addgene_192917 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-ELK1 | This paper | RRID:Addgene_192918 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-NR1H2 | This paper | RRID:Addgene_192919 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-TOX2 | This paper | RRID:Addgene_192920 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-LHX9 | This paper | RRID:Addgene_192921 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-ZFPM2 | This paper | RRID:Addgene_192922 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-HOPX | This paper | RRID:Addgene_192923 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-FOS | This paper | RRID:Addgene_192924 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-NR4A1 | This paper | RRID:Addgene_192925 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-RUNX2 | This paper | RRID:Addgene_192926 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-TAF4B | This paper | RRID:Addgene_192927 | Dox-inducible TF expression |
| Recombinant DNA reagent | pPB-cT3G-cERP2-RUNX1 | This paper | RRID:Addgene_192928 | Dox-inducible TF expression |
| Antibody | anti-AMHR2 (Rabbit IgG, polyclonal) | Thermo Fisher | Cat:PA5-13902; RRID:AB_2305463 | IF (1:100) |
| Antibody | anti-DAZL (Rabbit IgG, monoclonal) | Abcam | Cat:ab215718; RRID:AB_2893177 | IF (1:500) |
| Antibody | anti-FOXL2 (Goat IgG, polyclonal) | Novus | Cat:NB100-1277; RRID:AB_2106187 | IF (1:250) |
| Antibody | anti-44838 (Mouse IgG, monoclonal) | BD Bioscience | Cat:611202; RRID:AB_398736 | IF (1:200) |
| Antibody | anti-SOX17 (Goat IgG, polyclonal) | Novus | Cat:AF1924; RRID:AB_355060 | IF (1:500) |
| Antibody | anti-TFAP2C (Mouse IgG, monoclonal) | Abcam | Cat:ab110635; RRID:AB_10858471 | IF (1:250) |
| Antibody | anti-NR2F2 (Mouse IgG, monoclonal) | Novus | Cat:H7147; RRID:AB_1964214 | IF (1:250) |
| Antibody | anti-AMHR2-FITC (Rabbit IgG polyclonal) | Biorbyt | Cat:orb37457; RRID:AB_10992015 | Flow cytometry (1:60) |
| Antibody | anti-CD82-PerCP-Cy5.5 (Mouse IgG monoclonal) | BioLegend | Cat:342111; RRID:AB_2750124 | Flow cytometry (1:60) |
| Antibody | anti-EpCAM-APC-Cy7 (Mouse IgG monoclonal) |
BioLegend | Cat:324245; RRID:AB_2783193 | Flow cytometry (1:60) |
| Antibody | anti-FSHR-APC (Mouse IgG monoclonal) |
R&D Systems | Cat:FAB65591A; RRID:AB_2920602 | Flow cytometry (1:60) |
| Antibody | anti-Mouse IgG-AF647 (Donkey IgG polyclonal) |
Fisher | Cat:A31571; RRID:AB_162542 | IF secondary (1:250) |
| Antibody | anti-Goat IgG-AF568 (Donkey IgG polyclonal) |
Fisher | Cat:A11057; RRID:AB_2534104 | IF secondary (1:500) |
| Antibody | anti-Rabbit IgG-AF488 (Donkey IgG F(ab’)2 polyclonal) |
Jackson | Cat:711-546-152; RRID:AB_2340619 | IF secondary (1:500) |
| Commercial assay or kit |
Estradiol ELISA | DRG | Cat:EIA-2693 | |
| Commercial assay or kit |
Progesterone ELISA | DRG | Cat:EIA-1561 | |
| Commercial assay or kit | Evercode WT Mega scRNA-seq kit (v1) | Parse Biosciences | Cat:EC-W01050 | |
| Software, algorithm | Python scripts for data analysis |
This paper (Brixi et al., 2023) |
https://github.com/programmablebio/granulosa, (copy archived at swh:1:rev:3c650290779db376c4d1f3a14960b08b17ae5561) |
|
| Software, algorithm | Parse Biosciences barcode alignment pipeline |
Parse Biosciences | Version 0.9.6 |