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Gal4 binding is reduced ∼100-fold to binding sites extending past 30 bp into the nucleosome. (A–E) Ensemble FRET measurements of Gal4 binding to nucleosomes containing a ‘full’ Gal4 binding site at the indicated position. All binding curves are fit to a single binding isotherm. Error bars indicate ±1 SEM. (F) Summary of all S1/2 values from Figures 2 and 3. Error bars indicate ±1 SEM.
