Table 2.
Data collection and refinement statistics
| MutSα WT | MutSα V63E | MutSα WT ADP | |
|---|---|---|---|
| Data collection and processing | |||
| Microscope | Titan Krios | Titan Krios | Talos Arctica |
| Camera | K3 | K3 | K2 summit |
| Magnification | 105 000 | 105 000 | 130 000 |
| Voltage (kV) | 300 | 300 | 200 |
| Total dose (e−/Å2) | 50 | 50 | 60 |
| Defocus range (μm) | 0.9–2.4 | 0.9–2.4 | 1.6–3.2 |
| Number of fractions | 50 | 50 | 26 |
| Movies | 4821 | 4456 | 2608 |
| Pixel spacing (Å) | 0.836 | 0.836 | 1.041 |
| Symmetry imposed | C1 | C1 | C1 |
| Number of Mio particles, initial/final | 5.4/387 692 | 4.4/28 948 | 1.7/111 096 |
| Map resolution (Å) | 2.8 | 3.8 | 4.1 |
| FSC threshold | 0.143 | 0.143 | 0.143 |
| Map sharpening B factor (Å2) | Local (LocSpiral) | None | None |
| Model validation | |||
| Map pixel size (Å) | 0.839 | ||
| Real-space correlation (mask) | 0.85 | ||
| MolProbity score | 1.58 | ||
| Clash score | 5.11 | ||
| Ramachandran plot (%) | |||
| Favored | 96.51 | ||
| Allowed | 3.44 | ||
| Outliers | 0.05 | ||
| Ramachandran plot Z-score | −0.61 | ||
| Rotamer outliers (%) | 1.15 | ||
| Base pair geometry (rmsZbpG) | 0.93 | ||
| Mean model B-factors | |||
| Protein | 71.88 | ||
| DNA | 109.32 | ||
| ADP | 59.78 | ||