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Experimental validation of annotated non-ATG-started N-terminal extensions of the MAPRE2 and ZIC2 CDSs. (A) The schemes of the plasmids used for the experiment. (B) Results of the dual-luciferase reporter assay in HEK293T cells transfected with each plasmids for the indicated genes. The start-codons of the analyzed uORFs are listed below the gene name. Data are represented relative to the WT construct as mean ± SEM (*P < 0.05).
