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. 2022 Dec 5;54(1):1–14. doi: 10.1007/s42770-022-00877-0

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Fig. 6 — qRT-PCR analysis of the virulence gene expression in the presence of sodium chloride (NaCl) or sodium butyrate (But). The wild-type WT, ΔqseC, and ΔqseC/qseC + strains were cultured in low-glucose DMEM medium at 37 °C in the presence of 20 mM NaCl or But up to O.D of 1. Then, the RT-PCR assay was performed with the following genes: A ler and B stx-2. Statistical significance compared to the wild-type strain in the presence of NaCl. The bars without an asterisk showed no statistically significant difference, p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****) (two-way ANOVA)