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. 2022 Dec 5;54(1):1–14. doi: 10.1007/s42770-022-00877-0

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© The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Fig. 7 — Agar surface competition assay between Lactobacillus acidophilus (A) or Limosilactobacillus reuteri (B) and EHEC strains. The WT, ΔqseC, ΔqseC/qseC + , and L. acidophilus strains or L. reuteri in single culture (SC) were directly added to the agar surface. Then, the strain was mixed in co-culture (WT + L. acid or L. reuteri. and ΔqseC + L. acid or L. reuteri. and ΔqseC/qseC + + L. acid or L. reuteri) at the ratio of 1:1 or 1:5 of EHEC plus L. acid or L. reuteri, respectively, and added to the agar surface. After 16 h, the growth halo from the single-culture and co-culture plates was collected, diluted, plated, and counted. Bars without an asterisk showed no statistically significant difference, p < 0.001 (***), p < 0.0001 (****) (two-way ANOVA)