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Schematic representation of the pST1 eSpCas9(1.1)-P2A-EGFP plasmid vector
A pST1 plasmid vector containing rationally designed eSpCas9(1.1)3 linked to an enhanced green fluorescent protein (EGFP) reporter was designed for in vitro production of Cas9 mRNA. The plasmid vector representation was created with SnapGene.
