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. 2023 Feb 10;4(1):102112. doi: 10.1016/j.xpro.2023.102112

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© 2023 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Representative 1% agarose gel for quality control of in IVT eSpCas9(1.1)-P2A-EGFP mRNA

(1) A single stranded RNA ladder (ssRNA ladder) with a range of 500 bases to 9,000 bases is used for sizing the mRNA construct. (2) Resulting eSpCas9(1.1)-P2A-EGFP mRNA (mRNA Cas9 EGFP) has a length of 5500 nucleotides.