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Complete CTL medium
| Reagent | Final concentration | Amount |
|---|---|---|
| IMDM cell culture medium | 95% | 47.5 mL |
| hAB | 5% | 2.5 mL |
| rhIL-2 (10 UI/μL) | 50 UI/mL | 0.25 mL |
| rhIL-15 (1 ng/μL) | 10 ng/mL | 0.5 mL |
| Total | N/A | 50 mL |
Keep sterile, store at 4°C for up to 7 days; IL-2 degrades easily in the medium.
