Reagent | Final concentration | Amount |
---|---|---|
Nuclease-free UltraPure water | N/A | 3.2454 mL |
TRAC sgRNA (32,454 g/mol) | 1 μg/μL | 100 nmol |
Total | N/A | 3.2454 mL |
Make aliquots of 40 μL in nuclease-free tubes. Keep sterile, store at −20°C for up to 12 months.