| Reagent | Volume (1×) |
|---|---|
| Nuclease-free distilled water | Up to 20 μL |
| 2× NTP/CAP | 10 μL |
| 10× Reaction buffer | 2 μL |
| Linearized plasmid DNA template | 1 μg |
| T7 Enzyme Mix | 2 μL |
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