Figure 4.

Genome editing of somatic cells using JoinTRV

Plants were agroinoculated with an Agrobacterium strain that simultaneously hosts pLX-TRV1 and pLX-TRV2::sgPDS3-tFT, a pLX-TRV2 derivative with a sgRNA targeting two N. benthamiana PDS homeologs.

(A) Phenotypes of agroinoculated wild-type and Cas9 plants (∼1 month post inoculation).

(B) Severe photobleaching phenotype in Cas9 mature plants (∼2.5 months post inoculation).

(C) Chromatogram deconvolution of edited and control samples from agroinoculated Cas9 plants; note the high percentage of aberrant sequence downstream the predicted Cas9 cleavage site (blue dashed line).

(D) Total editing efficiency and indel distribution at one PDS locus of an edited sample (21 days post inoculation).