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. 2023 Feb 9;4(1):102091. doi: 10.1016/j.xpro.2023.102091

Lysogenic broth (LB)

Reagent Final concentration Amount
Tryptone 1.0% 10 g
Yeast extract 0.5% 5 g
NaCl 1.0% 10 g
Ultrapure water Up to 1 L
Total 1 L