Fragment	Sequence∗
sgPDS3-PVX	gaggtcagcaccagctagcaTTGGTAGTAGCGACTCCATGGTTTTAGAG CTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGA AAAAGTGGCACCGAGTCGGTGCATGTCTATAAATATAAGAGACC CTCTTATAGTAAGCAGAGTTGTTGGAGACGTTCTTGATCCGTTTA ATAGATCAATCACTCTAAAGGTTACTTATGGCCAAtagggtttgttaa gtttccc
sgPDS3-TRV2	cacttacccgagttaacgccTTGGTAGTAGCGACTCCATGGTTTTAGAGCT AGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAA AAGTGGCACCGAGTCGGTGCATGTCTATAAATATAAGAGACCCT CTTATAGTAAGCAGAGTTGTTGGAGACGTTCTTGATCCGTTTAAT AGATCAATCACTCTAAAGGTTACTTATGGCCAAatgtcccgaagaca ttaaac

∗ Cloning adapters for homology-based assembly are shown in lowercase, protospacer for targeting two N. benthamiana PDS homeologs in bold, the Cas9-binding scaffold is underlined and tFT is italicized.