Super optimal broth (SOB)
| Reagent | Final concentration | Amount |
|---|---|---|
| Tryptone | 2.0% | 20 g |
| Yeast extract | 0.5% | 5 g |
| NaCl | 10.0 mM | 0.584 g |
| KCl | 2.5 mM | 0.186 g |
| MgSO4 | 20.0 mM | 2.4 g |
| Ultrapure water | – | Up to 1 L |
| Total | – | 1 L |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.