Reagent | Final concentration | Amount |
---|---|---|
Guanidinium thiocyanate | 4 M | 47.3 g |
NaAcO pH 5.5 (3 M) | 0.1 M | 3.33 mL |
EDTA pH 8.0 (0.5 M) | 10 mM | 2 mL |
β-Mercaptoethanol | 0.1 M | 0.7 mL |
Ultrapure water | – | Up to 100 mL |
Total | – | 100 mL |
Reagent | Final concentration | Amount |
---|---|---|
Guanidinium thiocyanate | 4 M | 47.3 g |
NaAcO pH 5.5 (3 M) | 0.1 M | 3.33 mL |
EDTA pH 8.0 (0.5 M) | 10 mM | 2 mL |
β-Mercaptoethanol | 0.1 M | 0.7 mL |
Ultrapure water | – | Up to 100 mL |
Total | – | 100 mL |