Skip to main content
. 2023 Feb 9;4(1):102091. doi: 10.1016/j.xpro.2023.102091

RNA/DNA extraction buffer

Reagent Final concentration Amount
Guanidinium thiocyanate 4 M 47.3 g
NaAcO pH 5.5 (3 M) 0.1 M 3.33 mL
EDTA pH 8.0 (0.5 M) 10 mM 2 mL
β-Mercaptoethanol 0.1 M 0.7 mL
Ultrapure water Up to 100 mL
Total 100 mL