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. 2023 Feb 21;14(2):148. doi: 10.1038/s41419-023-05670-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Colorimetric quantification of m⁶A levels in HCT116 cells after METTL14 knockdown (P = 0.0322) or overexpression (P = 0.0012). B Relative expression of pri-miR-17, pre-miR-17 and miR-17-5p after METTL14 knockdown (P = 0.0335, 0.0313, 0.0322, 0.0002 respectively) or overexpression (P = 0.0001, 0.0084, 0.0121, 0.0049 respectively). C Possible m⁶A modification sites on the pri-miR-17 predicted by SRAMP database. D MeRIP and MeRIP-qPCR analysis demonstrating pri-miR-17 m⁶A modification in sh-NC and sh-METTL14 cells, P = 0.0009. E qPCR analysis of pri-miR-17 levels in different HCT116 cells groups after actinomycin D treatment. P (NC vs. METTL14 OE, 15 mins = 0.0272, 120 mins = 0.0021); P (si-NC vs. si-METTL14, 60 mins = 0.0209, 120 mins = 0.0175). Results shown were representative of at least 3 independent experiments. Statistical significance in (A), (B), (D), (E) was assessed by student’s t-test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Error bars, SD.