Fig. 5. METTL14 knockdown enhances pri-miR-17 mRNA stability via an m⁶A-YTHDC2-dependent pathway.

A Relative expression of pri-miR-17 after HCT116 cells transfected YTHDC1-2, YTHDF1-3 plasmids. P (NC vs. YTHDC1 OE) = 0.0198; P (NC vs. YTHDC2 OE) = 0.0385. B Relative expression of pri-miR-17 and miR-17-5p after HCT116 cells transfected YTHDC2 plasmids and cotransfected si-METTL14 and YTHDC2 plasmids. C PCR analysis of pri-miR-17 levels in different HCT116 cells groups after actinomycin D treatment. D Luciferase vector encoding the wild-type or m⁶A consensus sequence mutated pri-miR-17 were constructed. E YTHDC2 induced the posttranscriptional repression of pri-miR-17 in HCT116 cells. P (WT pri-miR-17: NC vs. YTHDC2 OE) = 0.0046. Results shown were representative of at least 3 independent experiments. Statistical significance in (E) was assessed by student’s t-test. Statistical significance in (A–C) was determined by one-way ANOVA with Dunnett’s multiple comparisons test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Error bars, SD. The grey value of protein bands has been quantified by Image J.