Infection with Pseudomonas aeruginosa alters airway functional parameters that remain unresolved in 6-month-old cystic fibrosis transmembrane conductance receptor knockout (KO) rats. Tracheae from wild-type (WT) and KO rats infected with P. aeruginosa were collected at 0, 3, 7, 14 and 28 days post-infection and imaged using micro-optical coherence tomography. Quantitation of the resultant images yielded airway surface liquid (ASL) at a) 2 months and b) 6 months; and mucociliary transport (MCT) at c) 2 months and d) 6 months of age. Data are presented as mean±sem. All data were analysed via two-way ANOVA with Sidak post-test. *: p<0.05, ***: p<0.001, ****: p<0.0001. n=6–8 per group.