Table 1.

Retention time, phytochemical compounds, and peak area % determined by GC–MS analysis of Nigella sativa seed extract.

Peak No.	Ret. Time	Phytochemical Compounds	Molecular Formula	Molecular Weight	Peak Area %
1	5.603	Glycerin	C3H8O3	92	0.47
2	18.622	Phenol, 2,4-bis(1,1-dimethylethyl)-	C14H22O	206	0.18
3	20.18	Phenol, 2,4-bis(1,1-dimethylethyl)-	C14H22O	206	0.82
4	24.244	Phenol, 2,4-bis(1,1-dimethylethyl)-	C14H22O	206	0.93
5	35.471	Hexadecanoic acid, methyl ester	C17H34O2	270	0.73
6	35.555	7,9-Di-tert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione	C17H24O3	276	0.15
7	35.807	Hexadecanoic acid, methyl ester	C17H34O2	270	0.52
8	36.575	l-(+)-Ascorbic acid 2,6-dihexadecanoate	C38H68O8	652	4.24
9	36.823	Pentadecanoic acid	C15H30O2	242	3.73
10	37.284	Hexadecanoic acid, ethyl ester	C17H34O2	270	0.82
11	37.511	Hexadecanoic acid, ethyl ester	C17H34O2	270	0.52
12	39.765	9,12-Octadecadienoic acid (Z,Z)-, methyl ester	C19H34O2	294	5.07
13	39.925	10,13-Eicosadienoic acid, methyl ester	C21H38O2	322	6.46
14	40.066	9-Octadecenoic acid (Z)-, methyl ester	C19H36O2	296	1.89
15	40.573	Methyl stearate	C19H38O2	298	0.23
16	41.125	Octadec-9-enoic acid	C18H34O2	282	47.64
17	41.386	9,12-Octadecadienoic acid, ethyl ester	C20H36O2	308	7.71
18	41.485	9,12-Octadecadienoic acid, ethyl ester	C20H36O2	308	16.71
19	42.183	Heptadecanoic acid, 15-methyl-, ethyl ester	C20H40O2	312	0.85
20	42.26	Heptadecanoic acid, 15-methyl-, ethyl ester	C20H40O2	312	0.32