Figure 6.

Concurrent in vivo RNA and protein packaging using Dps_MxT3. (A) Schematic of Dps_MxT3 concurrently packaging RNA and eGFP. RNA is packaged through interaction with the fused Dps-N peptide, while eGFP is loaded through specific targeting peptide (MxTP) interaction. (B) SDS-PAGE analysis of purified eGFP- and RNA-loaded Dps_MxT3. (C) Negative-stain TEM micrograph of purified eGFP- and RNA-loaded Dps_MxT3. (D) Native PAGE analysis showing Nat_MxT3 and eGFP- and RNA-loaded Dps_MxT3 via Coomassie blue staining (top), UV exposure for eGFP fluorescence analysis (middle), and GelRed staining for RNA detection (bottom).