Table 1.
Differential characteristics of strain GeG2T and type strains of related Novosphingobium species. Strains: 1. GeG2T; 2. N. rosa DSM 7285T (data from this study); 3. N. lotistagni THG-DN6.20T (data from [6]. All strains are positive for hydrolysis of aesculin; catalase; oxidase; assimilation of glucose, arabinose, and maltose; alkaline phosphatase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, β-galactosidase, α-glucosidase, and β-glucosidase activities. All strains are negative for hydrolysis of gelatin and urea; indol production; glucose fermentation; assimilation of mannitol, caprate, adipate, malate, citrate, phenylacetate; arginine dihydrolase, lipase (C14), and α-mannosidase activities. + , positive; − , negative; w, weakly positive; -* no growth after first transfer; ND, no data
| Characteristic | 1 | 2 | 3 |
|---|---|---|---|
| Isolation source | Cerrado soil | Rose rhizosphere | Lotus pond |
| Colony color | White | Yellow | Yellow |
| Cell size (μm): | |||
| Width | 0.3–0.6 | 0.3–0.5 | 0.6–0.8 |
| Length | 1.3–2.3 | 1.0–1.9 | 0.9–4.2 |
| Motility | + | + | - |
| Growth on TSA | - | + | w |
| Growth on NA | -* | + | + |
| Growth on LB | -* | + | - |
| Growth conditions: | |||
| pH | 4–7 | 4–7 | ND |
| NaCl (%) | 0–1 | 0–1.5 | 0–1 |
| Temperature (°C) | 15–33 | 15–33 | 4–42 |
| Facultatively anaerobic growth | + | - | - |
| Hydrolysis of starch | + | - | - |
| Nitrate reduction | w | - | - |
| Assimilation of: | |||
| Mannose | + | + | - |
| N-Acetylglucosamin | + | w | w |
| Gluconate | + | w | - |
| Enzyme activities of: | |||
| Esterase (C4) | w | + | w |
| Esterase lipase (C8) | w | w | w |
| Leucin-arylamidase | + | + | w |
| Valin-arylamidase | + | + | w |
| Cystin-arylamidase | w | w | w |
| Trypsin | - | - | + |
| Chymotrypsin | w | - | + |
| α-Galactosidase | - | - | + |
| β-Glucuronidase | w | - | + |
| N-Acetyl-β-glucosaminidase | - | w | - |
| α-Fucosidase | - | - | w |
| DNA G + C content (mol%) | 63.57 | 64.50 | 63.10 |