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. 2023 Feb 21;10(3):e200086. doi: 10.1212/NXI.0000000000200086

Figure 3. Changes of the 10-kDa Dextran, IgG, IgM, and Anti-MAG Antibody Permeability Evaluated Using an In Vitro Human BNB Coculture Model Exposed to Sera From Individuals With MAG Neuropathy.

Figure 3

PnMECs on the luminal side and human peripheral nerve pericytes on the abluminal side were cultured on a 24-well collagen-coated Transwell culture insert (pore size: 0.4 mm). MCDB 131 medium containing individual 10% sera from patients with anti-MAG neuropathy, patients with IgM-MG neuropathy, patients with ALS, and HCs were incubated with luminal side. The change in the 10-kDa dextran permeability coefficient (A), IgG permeability index (B), IgM permeability index (C), and MAG antibody (MAG-Ab) permeability (D) was calculated. Data are shown as the mean ± SEM. p Values were determined by a one-way ANOVA followed by the Tukey multiple comparison test (*p < 0.05, **p < 0.01, and ***p < 0.001 vs ALS or HC group followed by the Tukey multiple comparison test). ALS = amyotrophic lateral sclerosis; ANOVA = analysis of variance; BNB = blood-nerve barrier; IgM-MG = IgM–monoclonal gammopathy neuropathy; MAG = myelin-associated glycoprotein; PnMECs = peripheral nerve microvascular endothelial cells.