Skip to main content
. 2023 Feb 21;10(3):e200086. doi: 10.1212/NXI.0000000000200086

Figure 5. Effect of the Inhibition of TNF-α From Sera of Patients With MAG Neuropathy on IgM and MAG Antibody Permeability in the BNB Coculture Model.

Figure 5

TNF-α inhibition using TNF-α–neutralizing antibody from 2 10% sera with MAG neuropathy (MAG1 and MAG2) significantly decreased the IgM (A) and MAG antibody (B) permeability by approximately 50% in an in vitro human BNB coculture model consisting of both PnMECs on the luminal side and peripheral nerve pericytes on the abluminal side. After exposure to 10% sera from patients with IgM-MG (IgM-MG) or HCs, TNF-α–neutralizing antibody did not change the IgM/MAG antibody permeability. Data are shown as the mean ± SEM of 4 independent experiments. Statistical significance was determined by a paired 2-tailed t test. +TNF-α–neutralizing antibody, sera with TNF-α–neutralizing antibody. BNB = blood-nerve barrier; HC = healthy control; IgM-MG = IgM–monoclonal gammopathy neuropathy; MAG = myelin-associated glycoprotein; PnMECs = peripheral nerve microvascular endothelial cells; TNF = tumor necrosis factor.