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. 2023 Feb 9;8(1):74. doi: 10.3390/biomimetics8010074

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Procedure for the fabrication of highly organized 3D alginate scaffolds using a microfluidic device: (a) Production of hollow alginate microparticles using a two-channel fluid jacket microencapsulator; (b) gelation of alginate microparticles by ionic cross-linking with calcium ions; (c) obtainment of gelated alginate scaffold; (d) obtainment of alginate scaffolds with interconnected pores after treatment in a vacuum system overnight, to remove air bubbles. Adapted from [66] (a,d) and [67] (b,c).