MDA-MB-231 medium

Reagent	Final concentration	Amount
DMEM:F-12 for SILAC		450 mL
FBS dialyzed by ultracentrifugation 0.15 M NaCl	10%	50 mL
L-Arginine · 2HCl (13C6, 99%; 15N4, 99%)	1% 100 μg/mL	5 mL
L-Lysine · 2HCl (13C6, 99%; 15N2, 99%)	1% 100 μg/mL	5 mL
L-Leucine	1% 100 μg/mL	5 mL
Penicillin/Streptomycin	1%	5 mL
Puromycin dihydrochloride	0.5 μg/mL	250 μL
Total	N/A	520.25 mL