| Time (min) | Flow (μL/min) | % Mobile phase B |
|---|---|---|
| 0 | 0.300 | 2 |
| 8 | 0.300 | 2 |
| 134 | 0.300 | 35 |
| 134.1 | 0.300 | 90 |
| 138 | 0.300 | 90 |
| 138.1 | 0.300 | 2 |
| 149 | 0.300 | 2 |
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